anti p erk (Cell Signaling Technology Inc)

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Cell Signaling Technology Inc anti p erk
Anti P Erk, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 93/100, based on 15 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+p+bad/Phospho-Bad+Antibody+Sampler+Kit/pmc13023966-80-39-40
Average 93 stars, based on 15 article reviews

anti p erk - by Bioz Stars, 2026-10

93/100 stars

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Modification:

Article Title: Radioprotective effects of centipedegrass extract on NIH-3T3 fibroblasts via anti-oxidative activity
Article Snippet: A cell counting kit-8 was purchased from Sigma-Aldrich; Merck KGaA. .. Anti-AKT (#4691), anti-p-AKT (#4060), anti-ERK (#4695), anti-p-ERK (#4377), anti-p38 (#9212), anti-p-p38 (#9215), anti-JNK (#9252), anti-p-JNK (#9251), anti-caspase-3 (#9662), anti-cleaved caspase-3 (#9664), anti-Bcl-2 (#2870), anti-Bax (#5023), anti-Bad (#9292), anti-p-Bad (#9291), anti-poly(ADP-ribose) polymerase (PARP) (#9532), anti-cleaved PARP (#9541), anti-GAPDH (#2118), and anti-α-tubulin (#2144) were purchased from Cell Signaling Technology, Inc. Dulbecco's modified Eagle's medium (DMEM), penicillin/streptomycin (P/S), and fetal bovine serum (FBS) were purchased from Lonza. .. Annexin V and oxidative stress kits were purchased from Millipore.

other:

Article Title: A possible new activator of PI3K‐Huayu Qutan Recipe alleviates mitochondrial apoptosis in obesity rats with acute myocardial infarction
Article Snippet: Anti‐p‐Bad , Cell signaling technology , 5284S , 23 KDa , 1:1000.

Article Title: Tanshinone IIA Combined With Cyclosporine A Alleviates Lung Apoptosis Induced by Renal Ischemia-Reperfusion in Obese Rats
Article Snippet: Anti-p-Bad , Cell signaling technology , 5284S , 23 KDa , 1:1,000.

Incubation:

Article Title: MicroRNA-32-5p attenuates cerebral ischemia/reperfusion injuries by modulating the phosphatase and tensin homologous protein.
Article Snippet: Non-coding RNAs have recently attracted much attention with the potential in the treatment of cerebral ischemia/reperfusion (I/R) injuries.. In this study, we investigated the role of miR-32-5p in cerebral I/R injuries by using oxygen–glucose deprivation/reperfusion (OGD/R) PC12 cells and middle cerebral artery occlusion/reperfusion (MCAO/R) rats.. The expression of genes and proteins were detected by RT-qPCR and Western blot, respectively.

Article Title: Gypenoside Inhibits Endothelial Cell Apoptosis in Atherosclerosis by Modulating Mitochondria through PI3K/Akt/Bad Pathway.
Article Snippet: .. After being blocked in skim milk solution, the membrane was incubated overnight separately with antibodies anti-β-actin (Santa Cruz Biotechnology, Santa Cruz, CA, USA), anti-PI3K, anti-p-Akt, anti-p-Bad, anti-Cyt-c, anti-cleaved caspase 9, anti-cleaved caspase 3, anti-PARP, anti-DRP1, and anti-Mfn2 (Cell Signal, CST, USA). .. After that, the membrane was incubated with the secondary HRP-conjugated goat anti-rabbit antibodies (Santa Cruz Biotechnology).

Membrane:

Blocking Assay:

Article Title: Deficiency of kin17 Facilitates Apoptosis of Cervical Cancer Cells by Modulating Caspase 3, PARP, and Bcl-2 Family Proteins
Article Snippet: Then, the total 100 μ g of proteins were separated by 12% SDS-PAGE and transferred onto Immobilon®-P PVDF transfer membranes (EMD Millipore, Billerica, MA, USA). .. Following blocking with nonfat milk at room temperature for 1 h, the membranes were probed with anti-Kin17 (dilution, 1 : 500; cat. No. sc-32769; Santa Cruz Biotechnology, Inc.), anti-Bcl-xL (dilution, 1 : 1000; # 2764; Cell Signaling Technology, Inc.), anti-Bim (dilution, 1 : 1000; # 2933; Cell Signaling Technology, Inc.), anti-PARP (dilution, 1 : 1000; # 9542; Cell Signaling Technology, Inc.), anticleaved PARP (dilution, 1 : 1000; # 5625; Cell Signaling Technology, Inc.), anti-BAD (dilution, 1 : 1000; # 9239; Cell Signaling Technology, Inc.), anti-p-BAD (dilution, 1 : 1000; # 5284; Cell Signaling Technology, Inc.), and anti-GAPDH (dilution, 1 : 500; sc-47778; Santa Cruz Biotechnology, Inc.) monoclonal primary antibodies overnight at 4°C. .. After washing three times with TBST, HRP-conjugated secondary antibodies (dilution, 1 : 500; cat. No. sc-2318; Santa Cruz Biotechnology, Inc.) were used to develop immunoblots at room temperature for 1 h, which were processed using ab ECL-enhanced chemiluminescence substrate (Thermo Fisher Scientific, Inc.).

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